| Long name: |
Cy3 Conjugated, c-Raf (Ser259) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
c-Raf Ser259 |
| Specificity: |
This is a highly specific antibody against c-Raf Ser259 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser259 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser259 |
| Gene ID number: |
5894 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It is part of the Ras dependent signaling pathway from receptors to the nucleus, Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, bRaf, cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, phosphorylates MEK which in turn phosphorylates ERK, The Raf family of serine/threonine specific kinases is comprised of three members (aRaf |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
6430402F14Rik, AA990557, BB129353, C-RAF, Craf 1 transforming gene, Craf1, Craf1 transforming gene, D830050J10Rik, EC 2, MGC102375, Murine sarcoma 3611 oncogene 1, RAF, RAF1, Raf 1, Raf proto oncogene serine/threonine protein kinase, Raf-1, Raf1Ser259, c Raf, c-Raf, c-Raf, cRaf, murine leukemia viral v-raf-1 oncogene homolog 1 3611-MSV, proto-oncogene c-RAF, v-Raf, v-raf-1 murine leukemia viral oncogene homolog 1, vraf1 murine leukemia viral oncogene homolog 1, 1, 7, 11, RAF1phospho S259 |
| Properties: |
1 of the c-Raf (Ser259) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
c-Raf (Ser259) Conjugated |
| Short name: |
Conjugated, c-Raf (Ser259) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, c-Raf (Ser259) (Antibody to) |
| Alternative technique: |
antibodies |