| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BAP1 Ser592 |
| Specificity: |
BAP1 (Ser592), This phosphorylation site is homologous to the Ser590 site in Rat |
| Modification: |
Phosphorylation |
| Modification site: |
Ser592 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human BAP1 around the phosphorylation site of Ser592 |
| Gene ID: |
8314 |
| Swiss Prot: |
Q92560 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, Acts as a tumor suppressor, Catalytic component of the PR-DUB complex, Deubiquitination of HCFC1 does not lead to increase stability of HCFC1, Does not deubiquitinate monoubiquitinated histone H2B, Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination, It however does not mediate deubiquitination of BRCA1 and BARD1, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1), thereby protecting it from cytoplasmic sequestration (PubMed:24703950), with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains, Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
hucep-6 KIAA0272 UCHL2 |
| Also known as: |
BAP1 (Ser592) Polyclonal Antibody |
| Other name: |
Anti-BAP1 (Ser592) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BAP1 (Ser592) |
| Short name: |
Anti-BAP1 (Ser592) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-BAP1 (Ser592) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
950 |
| Gene: |
BAP1 |
More about : BAP1 |
| Long gene name: |
BRCA1 associated protein 1 |
| Synonyms gene name: |
BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) |
| Synonyms name: |
ubiquitin carboxy-terminal hydrolase |
| Locus: |
3p21, 1 |
| Discovery year: |
1998-09-17 |
| GenBank acession: |
AF045581 |
| Entrez gene record: |
8314 |
| Pubmed identfication: |
9528852 |
| Classification: |
Ubiquitin C-terminal hydrolases |
| Havana BLAST/BLAT: |
OTTHUMG00000158392 |
| Locus Specific Databases: |
LRG_529 |