| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CD163/M130 |
| Specificity: |
This antibody reacts specifically with CD163/M130 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CD163/M130 |
| Gene ID: |
9332 |
| Swiss Prot: |
Q86VB7 |
| Applications: |
IF(ICC), IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
After shedding, Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype, Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells, May play a role in the uptake and recycling of iron, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions, inositol triphosphate production and secretion of IL6 and CSF1, the soluble form (sCD163) may play an anti-inflammatory role, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme, Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
M130 MM130 SCARI1 |
| Also known as: |
CD163/M130 Polyclonal Antibody |
| Other name: |
Anti-CD163/M130 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CD163/M130 |
| Short name: |
Anti-CD163/M130 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CD163 molecule/M130 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CD163 and IDBG-16292 and ENSG00000177575 and 9332, CD163 and IDBG-639861 and ENSBTAG00000019669 and 533844, Cd163 and IDBG-186721 and ENSMUSG00000008845 and 93671, Extracellular, M130 and MM130, protein binding, this GO :0005044 and scavenger receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0006953 and acute-phase response and biological process this GO :0016020 and membrane and cellular component this GO :0030666 and endocytic vesicle membrane and cellular component, this GO :0005044 : scavenger receptor activity, this GO :0005044 : scavenger receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, CD163 molecule |
| Identity: |
1631 |
| Gene: |
CD163 |
More about : CD163 |
| Long gene name: |
CD163 molecule |
| Synonyms gene name: |
CD163 antigen |
| Locus: |
12p13, 31 |
| Discovery year: |
2000-01-18 |
| GenBank acession: |
Z22968 |
| Entrez gene record: |
9332 |
| Pubmed identfication: |
10403791 8370408 |
| RefSeq identity: |
NM_004244 NM_203416 |
| Classification: |
CD molecules Scavenger receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000168353 |