| Long name: |
Biotin Conjugated, CD163/M130 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CD163/M130 |
| Specificity: |
This is a highly specific antibody against CD163/M130 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
800-850/1156 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human CD163/M130 |
| Gene ID number: |
9332 |
| Swiss Prot: |
Q86VB7 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
After shedding, Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype, Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells, May play a role in the uptake and recycling of iron, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions, inositol triphosphate production and secretion of IL6 and CSF1, the soluble form (sCD163) may play an anti-inflammatory role, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme, Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
M130 MM130 SCARI1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CD163/M130 Conjugated |
| Short name: |
Conjugated, CD163/M130 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, CD163 molecule/M130 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CD163 and IDBG-16292 and ENSG00000177575 and 9332, CD163 and IDBG-639861 and ENSBTAG00000019669 and 533844, Cd163 and IDBG-186721 and ENSMUSG00000008845 and 93671, Extracellular, M130 and MM130, protein binding, this GO :0005044 and scavenger receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0006953 and acute-phase response and biological process this GO :0016020 and membrane and cellular component this GO :0030666 and endocytic vesicle membrane and cellular component, this GO :0005044 : scavenger receptor activity, this GO :0005044 : scavenger receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, CD163 molecule |
| Identity: |
1631 |
| Gene: |
CD163 |
More about : CD163 |
| Long gene name: |
CD163 molecule |
| Synonyms gene name: |
CD163 antigen |
| Locus: |
12p13, 31 |
| Discovery year: |
2000-01-18 |
| GenBank acession: |
Z22968 |
| Entrez gene record: |
9332 |
| Pubmed identfication: |
10403791 8370408 |
| RefSeq identity: |
NM_004244 NM_203416 |
| Classification: |
CD molecules Scavenger receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000168353 |