| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Axin1 |
| Specificity: |
This antibody reacts specifically with Axin1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Axin1 |
| Gene ID: |
8312 |
| Swiss Prot: |
O15169 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, Controls dorsoventral patterning via two opposing effects, Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7, Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation, In Wnt signaling, Likely to function as a tumor suppressor, and modulating Wnt-signaling, apoptosis and development, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway, down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B, Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PPP1R49 |
| Also known as: |
Axin1 Polyclonal Antibody |
| Other name: |
Anti-Axin1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Axin1 |
| Short name: |
Anti-Axin1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-axin 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
903 |
| Gene: |
AXIN1 |
More about : AXIN1 |
| Long gene name: |
axin 1 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 49 |
| Locus: |
16p13, 3 |
| Discovery year: |
1998-09-17 |
| GenBank acession: |
AF009674 |
| Entrez gene record: |
8312 |
| Pubmed identfication: |
9230313 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000064930 |