| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MDC1 |
| Specificity: |
This antibody reacts specifically with MDC1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MDC1 |
| Gene ID: |
9656 |
| Swiss Prot: |
Q14676 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1, Also required for downstream events subsequent to the recruitment of these proteins, CHEK1 and CHEK2 kinases, May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX, These include phosphorylation and activation of the ATM, and stabilization of TP53 and apoptosis, Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NFBD1 KIAA0170 Em:AB023051, 5 |
| Also known as: |
MDC1 Polyclonal Antibody |
| Other name: |
Anti-MDC1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MDC1 |
| Short name: |
Anti-MDC1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-mediator on Desoxyribonucleic acid-damage checkpoint 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
FHA domain binding, MDC1 and IDBG-634528 and ENSBTAG00000025526 and 511168, MDC1 and IDBG-76700 and ENSG00000137337 and 9656, Mdc1 and IDBG-179187 and ENSMUSG00000061607 and 240087, NFBD1, nuclei, this GO :0000724 and double-strand break repair via homolo this GO us recombination and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005694 and chromosome and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0006281 and DNA repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0031573 and intra-S DNA damage checkpoint and biological process this GO :0070975 and FHA domain binding and molecular function, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008022 : protein C-terminus binding and also this GO :0070975 : FHA domain binding, this GO :0008022 : protein C-terminus binding, this GO :0070975 : FHA domain binding, mediator of DNA-damage checkpoint 1 |
| Identity: |
21163 |
| Gene: |
MDC1 |
More about : MDC1 |
| Long gene name: |
mediator of DNA damage checkpoint 1 |
| Synonyms gene name: |
mediator of DNA-damage checkpoint 1 |
| Locus: |
6p21, 33 |
| Discovery year: |
2003-05-21 |
| GenBank acession: |
D79992 |
| Entrez gene record: |
9656 |
| Pubmed identfication: |
10975465 12607005 |
| RefSeq identity: |
NM_014641 |
| Havana BLAST/BLAT: |
OTTHUMG00000031075 |