| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
pan-Cytokeratin |
| Specificity: |
This antibody reacts specifically with pan-Cytokeratin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Intracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse Cytokeratin 19 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
4, 5, 6, Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification, Cytokeratins 1, Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin, It facilitates typing of normal, It is also useful in detecting micrometastases in lymph nodes, Synergy between the various components results in staining amplification, The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins, This enables identification of cells, Usually the type II cytokeratins are 8kD larger than their type I counterparts, a group comprising at least 29 different proteins, and 8 are members of the type II neutral to basic subfamily, are characteristic of epithelial and trichocytic cells, bone marrow and other tissues and for determining the origin of poorly differentiated tumors, lymphomas and neural tumors, metaplastic and neoplastic cells, which recognizes epitopes present in most human epithelial tissues, which would otherwise be stained only marginally, Cytokeratins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
pan-Cytokeratin, CK |
| Also known as: |
pan-Cytokeratin Polyclonal Antibody |
| Other name: |
Anti-pan-Cytokeratin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
pan-Cytokeratin |
| Short name: |
Anti-pan-Cytokeratin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-pan-Cytokeratin (polyclonal) |
| Alternative technique: |
polyclonals |