| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
pan Cytokeratin |
| Specificity: |
This antibody reacts specifically with pan Cytokeratin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human pan cytokeratin |
| Applications: |
IF(IHC-F), IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-F)(1:50-200), IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Pig/Swine (Sus scrofa), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
20 are epithelial genes and the remaining 10 are specific for trichocytes, Among them, Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, Cytokeratins interact with desmosomes and hemidesmosomes, In the cytoplasm, Numerous accessory proteins are involved in the genesis and maintenance of such structure, The cytokeratins are encoded by a family encompassing 30 genes, This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms, cell movement and differentiation, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
2, Cytokeratins, P-CK, [cytokeratins 1, pan CK, pan-CK, wide spectrum Cytokeratin, 4, 5, 6, 7, 8, 71, 72, 75, 78], pan-cytokeratin |
| Also known as: |
pan Cytokeratin Polyclonal Antibody |
| Other name: |
Anti-pan Cytokeratin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
pan Cytokeratin |
| Short name: |
Anti-pan Cytokeratin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-pan Cytokeratin (polyclonal) |
| Alternative technique: |
polyclonals |