| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Heparanase |
| Specificity: |
This antibody reacts specifically with Heparanase |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Secreted, Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Heparanase 50 kDa subunit |
| Gene ID: |
10855 |
| Swiss Prot: |
Q9Y251 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII, Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF, Enhances shedding of syndecans, Facilitates cell migration associated with metastasis, Heparin increases this AKT1/PKB activation, Implicated in hair follicle inner root sheath differentiation and hair homeostasis, Increases cell adhesion to the extacellular matrix (ECM), Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion, It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes, Participates in extracellular matrix (ECM) degradation and remodeling, Regulates osteogenesis, Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group, and increases endothelial invasion and angiogenesis in myelomas, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety, independent of its enzymatic activity, wound healing and inflammation, Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HPA2 HPR2 HPSE2 |
| Also known as: |
Heparanase Polyclonal Antibody |
| Other name: |
Anti-Heparanase Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Heparanase |
| Short name: |
Anti-Heparanase (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Heparanase (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12521 |
| Gene: |
UFS |
More about : UFS |
| Long gene name: |
heparanase 2 (inactive) |
| Synonyms gene name: |
urofacial syndrome heparanase 2 |
| Locus: |
10q24, 2 |
| Discovery year: |
2002-09-02 |
| GenBank acession: |
AF282885 |
| Entrez gene record: |
60495 |
| Pubmed identfication: |
11027606 20560210 9199567 20576607 |
| RefSeq identity: |
NM_021828 |
| Havana BLAST/BLAT: |
OTTHUMG00000018880 |