| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CHMP2A |
| Specificity: |
This antibody reacts specifically with CHMP2A |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CHMP2A |
| Gene ID: |
27243 |
| Swiss Prot: |
O43633 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
-I, ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released, Involved in HIV-1 p6- and p9-dependent virus release, MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, The ESCRT machinery also functions in topologically equivalent membrane fission events, The MVB pathway appears to require the sequential function of ESCRT-O, lysosomal enzymes and lipids, possibly in conjunction with the AAA ATPase VPS4, such as stimulated growth factor receptors, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses), -II and -III complexes, Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BC-2 CHMP2 VPS2 VPS2A |
| Also known as: |
CHMP2A Polyclonal Antibody |
| Other name: |
Anti-CHMP2A Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CHMP2A |
| Short name: |
Anti-CHMP2A (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CHMP2A (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30216 |
| Gene: |
CHMP2A |
More about : CHMP2A |
| Long gene name: |
charged multivesicular body protein 2A |
| Synonyms gene name: |
chromatin modifying protein 2A |
| Synonyms name: |
cerevisiae) , putative breast adenocarcinoma marker (32kD) VPS2 homolog A (S |
| Locus: |
19q13, 43 |
| Discovery year: |
2005-04-04 |
| GenBank acession: |
AF042384 |
| Entrez gene record: |
27243 |
| Pubmed identfication: |
15173323 11559748 |
| RefSeq identity: |
NM_014453 |
| Classification: |
Charged multivesicular body proteins ESCRT-III |
| Havana BLAST/BLAT: |
OTTHUMG00000183547 |