| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PARD6G |
| Specificity: |
This antibody reacts specifically with PARD6G |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PARD6G |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
PARD6G contains one OPR domain, PARD6G is found at highest levels in adult and fetal kidney, PARD6G participates in the linking of GTP-bound Rho small GTPases to atypical protein kinase C (PKC) proteins, The PDZ and pseudo-CRIB domains are required for interaction with Rho small GTPases, This assembly is involved in formation of normal tight junctions at epithelial cell-cell contacts, Though widely expressed, Through its complex formation with PARD3G, When atypical PKC and PARD6G are expressed with a constitutively active RAC, one PDZ (DHR) domain and one pseudo-CRIB domain, the proteins colocalize to the membrane ruffles, which are structures that occur at the leading edge of polarized cells during movement, PARD6G (partitioning defective 6 homolog gamma) is a 376 amino acid adaptor protein that is involved in cell polarization and asymmetrical cell division processes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PAR-6G PAR6gamma |
| Also known as: |
PARD6G Polyclonal Antibody |
| Other name: |
Anti-PARD6G Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PARD6G |
| Short name: |
Anti-PARD6G (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PARD6G (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
16076 |
| Gene: |
PARD6G |
More about : PARD6G |
| Long gene name: |
par-6 family cell polarity regulator gamma |
| Synonyms gene name: |
C, elegans) , par-6 (partitioning defective 6, elegans) homolog gamma par-6 partitioning defective 6 homolog gamma (C |
| Locus: |
18q23 |
| Discovery year: |
2001-07-31 |
| Entrez gene record: |
84552 |
| Pubmed identfication: |
11260256 |
| RefSeq identity: |
NM_032510 |
| Classification: |
PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132922 |