| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IKAP |
| Specificity: |
This antibody reacts specifically with IKAP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human IKAP |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activation of NFkB requires that IkB be phosphorylated on specific serine residues, IKAP (IKK-complex-associated protein) is a protein that acts as a scaffold, IKK alpha, IKK alpha and IKK beta and assembling them into an active kinase complex (7, IKK beta and IKK gamma (also designated NEMO), IKKalpha appears to be critical for NFkB activation in response to proinflammatory cytokines (4), IkB kinase alpha (IKK alpha), Phosphorylation of the IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), The functional IKK complex contains three subunits, and each appears to make essential contributions to IkB phosphorylation (6), interacting with NIK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation, previously designated CHUK (2), serines 32 and 36 (3), which itself is a central regulator for NFkB activation in response to TNF and IL-1 (5), which results in the targeted degradation of IkB (1), 8), The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
IKAP TOT1 ELP1 IKI3 |
| Also known as: |
IKAP Polyclonal Antibody |
| Other name: |
Anti-IKAP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IKAP |
| Short name: |
Anti-IKAP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-IKAP (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
5959 |
| Gene: |
IKBKAP |
More about : IKBKAP |
| Long gene name: |
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein |
| Synonyms gene: |
DYS |
| Synonyms gene name: |
dysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy type III) |
| Synonyms name: |
elongator acetyltransferase complex subunit 1 |
| Locus: |
9q31, 3 |
| Discovery year: |
1998-09-30 |
| GenBank acession: |
AF044195 |
| Entrez gene record: |
8518 |
| Pubmed identfication: |
9751059 11179008 |
| Classification: |
Elongator acetyltransferase complex |
| Havana BLAST/BLAT: |
OTTHUMG00000020465 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_251 |