| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPR77 |
| Specificity: |
This antibody reacts specifically with GPR77 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPF77 |
| Gene ID: |
27202 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
C5L2 is an integral membrane protein that belongs to the Gprotein-coupled receptor 1 family, C5L2 weakly couples to Gi-mediated signaling pathways and is widely expressed in the hippocampus, It is coexpressed with the C5a receptor (C5AR) on polymorphonuclear neutrophils and may modulate the activity of C5AR, Research suggests that C5L2 may be required for the in vitro release of high mobility group box 1 protein (HMGB1), The function of the C5a receptors, a chemotactic and inflammatory peptide, also designated G protein-coupled receptor 77, frontal cortex and liver, hypothalamus, is a receptor for C5a anaphylatoxin chemotactic receptor (C5L2), is still unclear, particularly that of C5L2, pons, Anaphylatoxin |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
C5L2 |
| Also known as: |
GPR77 Polyclonal Antibody |
| Other name: |
Anti-GPR77 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPR77 |
| Short name: |
Anti-GPR77 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-G protein-coupled receptor 77 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
GPR77 and IDBG-59470 and ENSG00000134830 and 27202, GPR77 and IDBG-639987 and ENSBTAG00000037735 and 522144, Gpr77 and IDBG-146283 and ENSMUSG00000074361 and 319430, Plasma membranes, protein binding, this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0004944 and C5a anaphylatoxin receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006935 and chemotaxis and biological process this GO :0006954 and inflammatory response and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0009925 and basal plasma membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0045087 and innate immune response and biological process this GO :0045177 and apical part of cell and cellular component this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0090024 and negative regulation of neutrophil chemotaxis and biological process this GO :1900165 and negative regulation of interleukin-6 secretion and biological process this GO :2000482 and regulation of interleukin-8 secretion and biological process, this GO :0004930 : G-protein coupled receptor activity, this GO :0004930 : G-protein coupled receptor activity and also this GO :0004944 : C5a anaphylatoxin receptor activity and also this GO :0005515 : protein binding, this GO :0004944 : C5a anaphylatoxin receptor activity, this GO :0005515 : protein binding, G protein-coupled receptor 77 |
| Identity: |
4527 |
| Gene: |
C5AR2 |
More about : C5AR2 |
| Long gene name: |
complement component 5a receptor 2 |
| Synonyms gene: |
GPR77 |
| Synonyms gene name: |
G protein-coupled receptor 77 |
| Locus: |
19q13, 32 |
| Discovery year: |
2000-01-20 |
| GenBank acession: |
AB038237 |
| Entrez gene record: |
27202 |
| Pubmed identfication: |
11165367 |
| RefSeq identity: |
NM_018485 |
| Classification: |
Complement component GPCRs |