| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GGA1 |
| Specificity: |
This antibody reacts specifically with GGA1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GGA1 |
| Gene ID: |
26088 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ARF (ADP-ribosylation factor), ARF-binding proteins) are ubiquitous coat proteins that facilitate the trafficking of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, GGA1, GGA1 and GGA2 do not associate with each other, GGAs have modular structures with an N-terminal VHS (VPS-27, Hrs, Members of the GGA family, The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster di-leucine (ACLL) sequences (3), The cytosolic domain of memapsin 2, The human GGA1 gene maps to chromosome 22 and encodes a protein that shares 45% sequence identity with GGA2 and GGA3 (1), a connecting hinge segment, and STAM) domain followed by a GAT (GGA and TOM1) domain, and a C-terminal GAE (?adaptin ear) domain (5), and clathrin (1?), are multidomain proteins that bind mannose 6-phosphate receptors (MPRs) (1, binds the VHS domains of GGA1 and GGA2 (6), but not that of memapsin 1, but they do colocalize on perinuclear membranes (2), 2, 4), GGA2 (also known as VEAR) and GGA3, The GGA family of proteins (Golgi-localized |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
GGA1 Polyclonal Antibody |
| Other name: |
Anti-GGA1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GGA1 |
| Short name: |
Anti-GGA1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GGA1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
17842 |
| Gene: |
GGA1 |
More about : GGA1 |
| Long gene name: |
ARF binding protein 1 , gamma adaptin ear containing, golgi associated |
| Locus: |
22q13, 1 |
| Discovery year: |
2001-12-19 |
| GenBank acession: |
AF190862 |
| Entrez gene record: |
26088 |
| Pubmed identfication: |
10747088 10747089 16407204 |
| RefSeq identity: |
NM_013365 |
| Classification: |
ARF binding proteins , Golgi associated, gamma adaptin ear containing |
| Havana BLAST/BLAT: |
OTTHUMG00000030985 |