| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GEM |
| Specificity: |
This antibody reacts specifically with GEM |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GEM |
| Gene ID: |
2669 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, Gem was also observed in developing trigeminal nerve ganglia in 12, High intracellular Gem levels, Rad and Gem bind calmodulin in a Ca2+-dependent manner via this C-terminal extension, The human Gem gene maps to chromosome &, The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface, demonstrating that Gem expression is a property of normal ganglionic development, involving residues 278â, promote profound changes in cell morphology, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons, which interact with intact microtubules and microfilaments, which lack typical C-terminal amino acid motifs for isoprenylation, #65279, &euro, &ldquo, 1, 297 in human Rad, 5 day mouse embryos, 8q22, Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIR |
| Also known as: |
GEM Polyclonal Antibody |
| Other name: |
Anti-GEM Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GEM |
| Short name: |
Anti-GEM (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GEM (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4234 |
| Gene: |
GEM |
More about : GEM |
| Long gene name: |
GTP binding protein overexpressed in skeletal muscle |
| Synonyms gene name: |
GTP-binding protein overexpressed in skeletal muscle |
| Synonyms name: |
kinase-inducible Ras-like protein |
| Locus: |
8q22, 1 |
| Discovery year: |
1995-08-15 |
| Entrez gene record: |
2669 |
| Pubmed identfication: |
7912851 11956230 |
| RefSeq identity: |
NM_181702 |
| Classification: |
RGK type GTPase family |
| Havana BLAST/BLAT: |
OTTHUMG00000164392 |