| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Frizzled 10/CD350 |
| Specificity: |
there is a chance that this antibody will react with this protein based on homology, Due to the similarity of this protein to Frizzled 9, Frizzled 10/CD350 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Frizzled 10/CD350 |
| Gene ID: |
11211 |
| Swiss Prot: |
Q9ULW2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, Both pathways seem to involve interactions with G-proteins, May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues, Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes, which leads to the activation of disheveled proteins, Receptor for Wnt proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CD350 |
| Also known as: |
Frizzled 10/CD350 Polyclonal Antibody |
| Other name: |
Anti-Frizzled 10/CD350 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Frizzled 10/CD350 |
| Short name: |
Anti-Frizzled 10/CD350 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Frizzled 10/CD350 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4039 |
| Gene: |
FZD10 |
More about : FZD10 |
| Long gene name: |
frizzled class receptor 10 |
| Synonyms gene name: |
frizzled (Drosophila) homolog 10 frizzled homolog 10 (Drosophila) frizzled 10, seven transmembrane spanning receptor frizzled family receptor 10 |
| Locus: |
12q24, 33 |
| Discovery year: |
1999-09-09 |
| GenBank acession: |
AB027464 |
| Entrez gene record: |
11211 |
| Pubmed identfication: |
10448064 |
| Classification: |
Class F frizzled CD molecules , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000168380 |