| Long name: |
Biotin Conjugated, Frizzled 10/CD350 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Frizzled 10/CD350 |
| Specificity: |
Due to the similarity of this protein to Frizzled 9, there is a chance that this antibody will react with this protein based on homology, This is a highly specific antibody against Frizzled 10/CD350 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
100-150/581 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Frizzled 10/CD350 |
| Gene ID number: |
11211 |
| Swiss Prot: |
Q9ULW2 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, Both pathways seem to involve interactions with G-proteins, May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues, Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes, which leads to the activation of disheveled proteins, Receptor for Wnt proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
CD350 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Frizzled 10/CD350 Conjugated |
| Short name: |
Conjugated, Frizzled 10/CD350 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Frizzled 10/CD350 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4039 |
| Gene: |
FZD10 |
More about : FZD10 |
| Long gene name: |
frizzled class receptor 10 |
| Synonyms gene name: |
frizzled (Drosophila) homolog 10 frizzled homolog 10 (Drosophila) frizzled 10, seven transmembrane spanning receptor frizzled family receptor 10 |
| Locus: |
12q24, 33 |
| Discovery year: |
1999-09-09 |
| GenBank acession: |
AB027464 |
| Entrez gene record: |
11211 |
| Pubmed identfication: |
10448064 |
| Classification: |
Class F frizzled CD molecules , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000168380 |