| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HFE/Hemochromatosis |
| Specificity: |
This antibody reacts specifically with HFE/Hemochromatosis |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HFE/Hemochromatosis |
| Gene ID: |
3077 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Immunocytochemistry shows that the HFE protein and TfR both are expressed in the crypt enterocytes, In placenta, It is proposed that HFE has two mutually exclusive activities in cells: inhibition of uptake or inhibition of release of iron and that the balance between serum transferrin saturation and serum transferrin-receptor concentrations determines which of these functions predominates, Since hemochromatosis is a relatively easily treated disorder if diagnosed, The HFE protein, The gene which encodes HFE maps to human chromosome 6p21, Western blots show that, and heart failure, as is the case in human placenta, diabetes, hypermelanotic pigmentation of the skin, normally is expressed in crypt enterocytes of the duodenum where it has a unique, predominantly intracellular localization, providing a link between the HFE protein and iron transport, the HFE protein co-localizes with and forms a stable association with the transferrin receptor (TfR), the HFE protein in crypt enterocytes is physically associated with the TfR and with Î, this is a form of preventable cancer, which is defective in hereditary hemo-chromatosis, ², 2-microglobulin, 3, The features of hemochromatosis include cirrhosis of the liver |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HLA-H |
| Also known as: |
HFE/Hemochromatosis Polyclonal Antibody |
| Other name: |
Anti-HFE/Hemochromatosis Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HFE/Hemochromatosis |
| Short name: |
Anti-HFE/Hemochromatosis (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-hemochromatosis/Hemochromatosis (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, HFE and IDBG-67372 and ENSG00000010704 and 3077, HFE1 and HH and HLA-H and MVCD7 and TFQTL2, Hfe and IDBG-136751 and ENSMUSG00000006611 and 15216, Plasma membranes, peptide antigen binding, this GO :0001916 and positive regulation of T cell mediated cytotoxicity and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005769 and early endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006461 and protein complex assembly and biological process this GO :0006879 and cellular iron ion homeostasis and biological process this GO :0006955 and immune response and biological process this GO :0007565 and female pregnancy and biological process this GO :0010106 and cellular response to iron ion starvation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0019882 and antigen processing and presentation and biological process this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0042446 and hormone biosynthetic process and biological process this GO :0042605 and peptide antigen binding and molecular function this GO :0042612 and MHC class I protein complex and cellular component this GO :0045177 and apical part of cell and cellular component this GO :0045178 and basal part of cell and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0055037 and recycling endosome and cellular component this GO :0055072 and iron ion homeostasis and biological process this GO :0060586 and multicellular organismal iron ion homeostasis and biological process this GO :0097459 and iron ion import into cell and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0042605 : peptide antigen binding, this GO :0005515 : protein binding, this GO :0042605 : peptide antigen binding, 37142 and IDBG-635416 and ENSBTAG00000013065 and 497622, hemochromatosis |
| Identity: |
4886 |
| Gene: |
HFE |
More about : HFE |
| Long gene name: |
hemochromatosis |
| Synonyms name: |
high Fe |
| Locus: |
6p22, 2 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
3077 |
| Pubmed identfication: |
3460331 |
| Classification: |
C1-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000016348 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database LRG_748 |