| Long name: |
FITC Conjugated, HFE/Hemochromatosis Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HFE/Hemochromatosis |
| Specificity: |
This is a highly specific antibody against HFE/Hemochromatosis |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human HFE/Hemochromatosis |
| Gene ID number: |
3077 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Immunocytochemistry shows that the HFE protein and TfR both are expressed in the crypt enterocytes, In placenta, It is proposed that HFE has two mutually exclusive activities in cells: inhibition of uptake or inhibition of release of iron and that the balance between serum transferrin saturation and serum transferrin-receptor concentrations determines which of these functions predominates, Since hemochromatosis is a relatively easily treated disorder if diagnosed, The HFE protein, The gene which encodes HFE maps to human chromosome 6p21, Western blots show that, and heart failure, as is the case in human placenta, diabetes, hypermelanotic pigmentation of the skin, normally is expressed in crypt enterocytes of the duodenum where it has a unique, predominantly intracellular localization, providing a link between the HFE protein and iron transport, the HFE protein co-localizes with and forms a stable association with the transferrin receptor (TfR), the HFE protein in crypt enterocytes is physically associated with the TfR and with Î, this is a form of preventable cancer, which is defective in hereditary hemo-chromatosis, ², 2-microglobulin, 3, The features of hemochromatosis include cirrhosis of the liver |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HLA-H |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HFE/Hemochromatosis Conjugated |
| Short name: |
FITC Conjugated, HFE/Hemochromatosis Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, hemochromatosis/Hemochromatosis (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, HFE and IDBG-67372 and ENSG00000010704 and 3077, HFE1 and HH and HLA-H and MVCD7 and TFQTL2, Hfe and IDBG-136751 and ENSMUSG00000006611 and 15216, Plasma membranes, peptide antigen binding, this GO :0001916 and positive regulation of T cell mediated cytotoxicity and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005769 and early endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006461 and protein complex assembly and biological process this GO :0006879 and cellular iron ion homeostasis and biological process this GO :0006955 and immune response and biological process this GO :0007565 and female pregnancy and biological process this GO :0010106 and cellular response to iron ion starvation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0019882 and antigen processing and presentation and biological process this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0042446 and hormone biosynthetic process and biological process this GO :0042605 and peptide antigen binding and molecular function this GO :0042612 and MHC class I protein complex and cellular component this GO :0045177 and apical part of cell and cellular component this GO :0045178 and basal part of cell and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0055037 and recycling endosome and cellular component this GO :0055072 and iron ion homeostasis and biological process this GO :0060586 and multicellular organismal iron ion homeostasis and biological process this GO :0097459 and iron ion import into cell and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0042605 : peptide antigen binding, this GO :0005515 : protein binding, this GO :0042605 : peptide antigen binding, 37142 and IDBG-635416 and ENSBTAG00000013065 and 497622, hemochromatosis |
| Identity: |
4886 |
| Gene: |
HFE |
More about : HFE |
| Long gene name: |
hemochromatosis |
| Synonyms name: |
high Fe |
| Locus: |
6p22, 2 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
3077 |
| Pubmed identfication: |
3460331 |
| Classification: |
C1-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000016348 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database LRG_748 |