| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MPP5/PALS1 |
| Specificity: |
This antibody reacts specifically with MPP5/PALS1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MPP5/PALS1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Because tumors exhibit perturbations in epithelial polarity, Loss of Pals1 function results in delayed polarization, Pals1, Pals1 presents a new potential target in the study of carcinogenesis, Tight junctions assist in the formation of polarity in the epithelia by establishing a barrier to separate apical and basolateral membranes, decreased transepithelial electrical resistance and an inability to form lumenal cysts, importantly, mediates interaction between the two complexes via interaction with Par6, the Crumbs-Pals1-Patj complex and the Cdc42-Par6-Par3-aPKC complex, Two highly conserved complexes are responsible for the assembly of tight junctions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PALS1 FLJ12615 |
| Also known as: |
MPP5/PALS1 Polyclonal Antibody |
| Other name: |
Anti-MPP5/PALS1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MPP5/PALS1 |
| Short name: |
Anti-MPP5/PALS1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MPP5/PALS1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
18669 |
| Gene: |
MPP5 |
More about : MPP5 |
| Long gene name: |
membrane palmitoylated protein 5 |
| Synonyms gene name: |
membrane protein, palmitoylated 5 , palmitoylated 5 (MAGUK p55 subfamily member 5) membrane protein |
| Synonyms name: |
stardust protein associated with Lin-7 1 |
| Locus: |
14q23, 3 |
| Discovery year: |
2002-05-23 |
| GenBank acession: |
AK022677 |
| Entrez gene record: |
64398 |
| Pubmed identfication: |
11927608 |
| RefSeq identity: |
NM_022474 |
| Classification: |
Crumbs complex Membrane associated guanylate kinases PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000171243 |