| Long name: |
Biotin Conjugated, MPP5/PALS1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MPP5/PALS1 |
| Specificity: |
This is a highly specific antibody against MPP5/PALS1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human MPP5/PALS1 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Because tumors exhibit perturbations in epithelial polarity, Loss of Pals1 function results in delayed polarization, Pals1, Pals1 presents a new potential target in the study of carcinogenesis, Tight junctions assist in the formation of polarity in the epithelia by establishing a barrier to separate apical and basolateral membranes, decreased transepithelial electrical resistance and an inability to form lumenal cysts, importantly, mediates interaction between the two complexes via interaction with Par6, the Crumbs-Pals1-Patj complex and the Cdc42-Par6-Par3-aPKC complex, Two highly conserved complexes are responsible for the assembly of tight junctions |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
PALS1 FLJ12615 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MPP5/PALS1 Conjugated |
| Short name: |
Conjugated, MPP5/PALS1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, MPP5/PALS1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
18669 |
| Gene: |
MPP5 |
More about : MPP5 |
| Long gene name: |
membrane palmitoylated protein 5 |
| Synonyms gene name: |
membrane protein, palmitoylated 5 , palmitoylated 5 (MAGUK p55 subfamily member 5) membrane protein |
| Synonyms name: |
stardust protein associated with Lin-7 1 |
| Locus: |
14q23, 3 |
| Discovery year: |
2002-05-23 |
| GenBank acession: |
AK022677 |
| Entrez gene record: |
64398 |
| Pubmed identfication: |
11927608 |
| RefSeq identity: |
NM_022474 |
| Classification: |
Crumbs complex Membrane associated guanylate kinases PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000171243 |