| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EBF2 |
| Specificity: |
This antibody reacts specifically with EBF2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EBF2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
EBF2 forms a homodimer or a heterodimer with a related family member, EBF2 is a transcription factor which, Lack of EBF2 has been found to cause a small defect in the terminal differentiation of osteoblasts, Localized to the nucleus, OPG, OPG, activates the decoy receptor for RANKL, along with reduced bone mass and an increase in osteoclasts, in osteoblasts, in synergy with the Wnt-responsive LEF1/CTNNB1 pathway, in turn, regulates osteoclast differentiation, whose members are all helix-loop-helix transcription factors, Early B-cell factor 2 is a 575 amino acid protein belonging to the COE family of proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ11500 COE2 |
| Also known as: |
EBF2 Polyclonal Antibody |
| Other name: |
Anti-EBF2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EBF2 |
| Short name: |
Anti-EBF2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-early B-cell factor 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0035563 and positive regulation of chromatin binding and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0046983 and protein dimerization activity and molecular function this GO :0050873 and brown fat cell differentiation and biological process this GO :0060612 and adipose tissue development and biological process, EBF2 and IDBG-281524 and ENSG00000221818 and 64641, Ebf2 and IDBG-175890 and ENSMUSG00000022053 and 13592, nuclei, protein dimerization activity, this GO :0001709 and cell fate determination and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006351 and transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0005515 : protein binding and also this GO :0046872 : metal ion binding and also this GO :0046983 : protein dimerization activity, this GO :0003682 : chromatin binding, this GO :0005515 : protein binding, this GO :0046872 : metal ion binding, this GO :0046983 : protein dimerization activity, 59602 and IDBG-635598 and ENSBTAG00000040390 and, early B-cell factor 2 |
| Identity: |
19090 |
| Gene: |
EBF2 |
More about : EBF2 |
| Long gene name: |
early B-cell factor 2 |
| Locus: |
8p21, 2 |
| Discovery year: |
2002-08-09 |
| GenBank acession: |
AK021562 AK001144 |
| Entrez gene record: |
64641 |
| Pubmed identfication: |
9151732 |
| RefSeq identity: |
NM_022659 |
| Havana BLAST/BLAT: |
OTTHUMG00000163838 |