| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Doppel/DPL |
| Specificity: |
This antibody reacts specifically with Doppel/DPL |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Doppel |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
BSE in cattle, Dpl does not appear to contribute to prion disease progression, Dpl is also thought to play a role in angiogenesis, Dpl is characterized by an alpha-helical conformation, Dpl is highly expressed in adult testis and heart and is detectable in the brain of neonatal mice, Infectious PrPSc is highly expressed in the brain of animals affected by TSEs, The PRND gene locus, and Cruetzfeldt-Jacob disease in humans, and it is presented on the cell surface by a glycosylphosphatidylinositol anchor, and two N-linked oligosaccharides, but ectopic expression of Dpl is implicated in neuronal degeneration of ataxic PRP-deficient mice, encodes for the doppel protein (Dpl), including scrapie in sheep, infectious or sporadic, intramolecular disulfide bonds, lethal neurodegenerative disorders involving alterations of the prion protein (PrP), located on human chromosome 20p, specifically maturation of the blood-brain barrier, which exhibits approximately 25% sequence homology with PrP, Prion diseases or transmissible spongiform encephalopathies (TSEs) are manifested as genetic |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DPL dJ1068H6, 4 DOPPEL PrPLP |
| Also known as: |
Doppel/DPL Polyclonal Antibody |
| Other name: |
Anti-Doppel/DPL Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Doppel/DPL |
| Short name: |
Anti-Doppel/DPL (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Doppel/DPL (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
15748 |
| Gene: |
PRND |
More about : PRND |
| Long gene name: |
prion like protein doppel |
| Synonyms gene name: |
prion protein 2 (dublet) |
| Synonyms name: |
prion-like protein doppel downstream prion protein-like gene |
| Locus: |
20p13 |
| Discovery year: |
2001-05-30 |
| GenBank acession: |
AF106918 |
| Entrez gene record: |
23627 |
| Pubmed identfication: |
10525406 10577243 |
| RefSeq identity: |
NM_012409 |
| Havana BLAST/BLAT: |
OTTHUMG00000031789 |