| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CRBN |
| Specificity: |
This antibody reacts specifically with CRBN |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CRBN |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CRBN acts as a protease in mitochondria and is thought to regulate the assembly of KCNT1, CRBN functions are thought to be related to energy metabolism, Defects in the CRBN gene are associated with mild mental retardation, Localized to the cytoplasm, The gene encoding CRBN belongs to a family of ATP-dependent lon proteases that play a role in membrane trafficking and proteolysis, as well as the surface expression of KCNT1 in brain regions known to affect memory and learning, learning and memory, such as the hippocampus, CRBN is a 442 amino acid protein which is highly concentrated in human brain tissue |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MRT2 |
| Also known as: |
CRBN Polyclonal Antibody |
| Other name: |
Anti-CRBN Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CRBN |
| Short name: |
Anti-CRBN (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CRBN (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30185 |
| Gene: |
CRBN |
More about : CRBN |
| Long gene name: |
cereblon |
| Synonyms gene: |
MRT2A |
| Synonyms gene name: |
2A , autosomal recessive, mental retardation, non-syndromic |
| Locus: |
3p26, 2 |
| Discovery year: |
2004-05-19 |
| GenBank acession: |
BC017419 |
| Entrez gene record: |
51185 |
| Pubmed identfication: |
15557513 |
| RefSeq identity: |
NM_016302 |
| Havana BLAST/BLAT: |
OTTHUMG00000090261 |