| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RNF60/Midline-2 |
| Specificity: |
This antibody reacts specifically with RNF60/Midline-2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RNF60/Midline-2 |
| Gene ID: |
26040 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Dimerization is mediated by the tripartite motif (RBCC, It also interacts with IGBP1 (Lymphocyte signaling protein A4), Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1, Midline-2 is expressed at low levels in fetal kidney and lung, RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30, and in adult prostate, one B30, one COS domain, one fibronectin type-III domain and one RING-type zinc finger, ovary and small intestine, 2 domain, 2/SPRY domain, Midline-2 is a 715 amino acid protein encoded by the human gene MID2 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FXY2 TRIM1 RNF60 MRX101 |
| Also known as: |
RNF60/Midline-2 Polyclonal Antibody |
| Other name: |
Anti-RNF60/Midline-2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RNF60/Midline-2 |
| Short name: |
Anti-RNF60/Midline-2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RNF60/Midline-2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7096 |
| Gene: |
MID2 |
More about : MID2 |
| Long gene name: |
midline 2 |
| Locus: |
Xq22, 3 |
| Discovery year: |
1999-08-25 |
| Entrez gene record: |
11043 |
| Pubmed identfication: |
10400986 |
| RefSeq identity: |
NM_012216 |
| Classification: |
X-linked mental retardation Tripartite motif containing Ring finger proteins Fibronectin type III domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000022171 |
| Locus Specific Databases: |
Mental Retardation database |