| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Midline-1 |
| Specificity: |
This antibody reacts specifically with Midline 1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Midline-1/RNF59 |
| Gene ID: |
4281 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in MID1 are the cause of Opitz syndrome type I (OS-I), It also interacts with IGBP1 (Lymphocyte signaling protein A4), It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2, Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation, OS-I is an X-linked recessive disorder characterized by hypertelorism, OS-I mutations produce proteins with a decreased affinity for microtubules, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1, developmental delay and congenital heart defects, genital-urinary defects such as hypospadias in males and splayed labia in females, imperforate anus, lip-palate-laryngotracheal clefts, one B30, one COS domain, one fibronectin type-III domain and one RING-type zinc finger, 2/SPRY domain, Midline-1 (Tripartite motif-containing protein 18 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
OS FXY TRIM18 RNF59 |
| Also known as: |
Midline 1 Polyclonal Antibody |
| Other name: |
Anti-Midline 1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Midline 1 |
| Short name: |
Anti-Midline 1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Midline 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7095 |
| Gene: |
MID1 |
More about : MID1 |
| Long gene name: |
midline 1 |
| Synonyms name: |
Opitz/BBB syndrome |
| Locus: |
Xp22 |
| Discovery year: |
1997-12-12 |
| GenBank acession: |
Y13667 |
| Pubmed identfication: |
9354791 9425238 |
| Classification: |
Tripartite motif containing Ring finger proteins Fibronectin type III domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000021127 |
| Locus Specific Databases: |
Mental Retardation database |