| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
AKR1C1/2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This AKR1C1/2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P52895, Q04828 |
| Purity: |
Antigen affinity |
| Description: |
AKR1C1 binds bile acid with high affinity, The AKR1C1 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14, The enzymes display overlapping but distinct substrate specificity, These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors, Three transcript variants encoding two different isoforms have been found for this gene, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity, which consists of more than 40 known enzymes and proteins, AKR1C1 and AKR1C2 are members of the aldo/keto reductase superfamily |
| Immunogen: |
Amino acids M1-K123 from human AKR1C1 were used as the immunogen for the AKR1C1/2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the AKR1C1/2 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
AKR1C1/2 |
| Short name: |
AKR1C1/2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
AKR1C1/2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
384 |
| Gene: |
AKR1C1 |
More about : AKR1C1 |
| Long gene name: |
aldo-keto reductase family 1 member C1 |
| Synonyms gene: |
DDH1 |
| Synonyms gene name: |
20-alpha (3-alpha)-hydroxysteroid dehydrogenase) , aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1 |
| Synonyms: |
DDH MBAB DD1 HAKRC |
| Synonyms name: |
20-alpha (3-alpha)-hydroxysteroid dehydrogenase , dihydrodiol dehydrogenase 1 |
| Locus: |
10p15, 1 |
| Discovery year: |
1994-09-14 |
| GenBank acession: |
D26124 |
| Entrez gene record: |
1645 |
| Pubmed identfication: |
8011662 |
| RefSeq identity: |
NM_001353 |
| Classification: |
Aldo-keto reductases |
| Havana BLAST/BLAT: |
OTTHUMG00000017580 |