| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Collagen IV |
| Specificity: |
Collagen IV, This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Secreted |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain |
| Gene ID: |
12826 |
| Swiss Prot: |
P02463 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Arresten, Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation, Ligand for alpha1/beta1 integrin (By similarity), Specifically inhibits endothelial cell proliferation, The C-terminal half is found to possess the anti-angiogenic activity, comprising the C-terminal NC1 domain, forming a 'chicken-wire' meshwork together with laminins, inhibits angiogenesis and tumor formation, migration and tube formation, proteoglycans and entactin/nidogen, Type IV collagen is the major structural component of glomerular basement membranes (GBM) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ22259 DKFZp686I14213 |
| Also known as: |
Collagen IV Polyclonal Antibody |
| Other name: |
Anti-Collagen IV Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Collagen IV |
| Short name: |
Anti-Collagen IV (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Collagen IV (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2203 |
| Gene: |
COL4A2 |
More about : COL4A2 |
| Long gene name: |
collagen type IV alpha 2 chain |
| Synonyms gene name: |
collagen type IV alpha 2 |
| Synonyms name: |
canstatin collagen type IV alpha 2 |
| Locus: |
13q34 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AK025912 |
| Entrez gene record: |
1284 |
| Pubmed identfication: |
2439508 3025878 |
| RefSeq identity: |
NM_001846 |
| Classification: |
Collagens |
| Havana BLAST/BLAT: |
OTTHUMG00000017344 |