| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Collagen III |
| Specificity: |
it will only recognize the full length Collagen III protein and not the mature form, Collagen III, This antibody is produced with an immunogen contained within the COLFI propeptide region |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Secreted |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain (C-terminal propeptide) |
| Gene ID: |
1281 |
| Swiss Prot: |
P02461 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Involved in regulation of cortical development, Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12, Collagen type III occurs in most soft connective tissues along with type I collagen |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
C-P4Halpha(III) |
| Also known as: |
Collagen III Polyclonal Antibody |
| Other name: |
Anti-Collagen III Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Collagen III |
| Short name: |
Anti-Collagen III (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Collagen III (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30135 |
| Gene: |
P4HA3 |
More about : P4HA3 |
| Long gene name: |
prolyl 4-hydroxylase subunit alpha 3 |
| Synonyms gene name: |
2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III , alpha polypeptide III prolyl 4-hydroxylase, procollagen-proline |
| Synonyms name: |
collagen prolyl 4-hydroxylase alpha(III) |
| Locus: |
11q13, 4 |
| Discovery year: |
2004-05-12 |
| GenBank acession: |
AY327887 |
| Entrez gene record: |
283208 |
| Pubmed identfication: |
14500733 |
| RefSeq identity: |
NM_182904 |
| Havana BLAST/BLAT: |
OTTHUMG00000165254 |