| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
WNK4 |
| Specificity: |
This antibody reacts specifically with WNK4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human WNK4 |
| Gene ID: |
65266 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also inhibits the renal K(+)channel, KCNJ1, SLC12A3, WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+)secretion to maintain integrated homeostasis, by phosphorylation which appears to prevent membrane trafficking of SLC12A3, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis, Regulates the activity of the thiazide-sensitive Na-Cl cotransporter |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
WNK4 Polyclonal Antibody |
| Other name: |
Anti-WNK4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
WNK4 |
| Short name: |
Anti-WNK4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-WNK lysine deficient protein kinase 4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Cell surfaces, WNK4 and IDBG-51675 and ENSG00000126562 and 102724304, WNK4 and IDBG-640438 and ENSBTAG00000019907 and 100849076, Wnk4 and IDBG-211994 and ENSMUSG00000035112 and 69847, this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005923 and tight junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006811 and ion transport and biological process this GO :0006821 and chloride transport and biological process this GO :0008104 and protein localization and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0019869 : chloride channel inhibitor activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019869 : chloride channel inhibitor activity, transferring phosphorus-containing groups and molecular function this GO :0019869 and chloride channel inhibitor activity and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0050794 and regulation of cellular process and biological process this GO :0050801 and ion homeostasis and biological process this GO :0070294 and renal sodium ion absorption and biological process this GO :0072156 and distal tubule morphogenesis and biological process this GO :0090188 and negative regulation of pancreatic juice secretion and biological process, 65266, WNK lysine deficient protein kinase 4 |
| Identity: |
14544 |
| Gene: |
WNK4 |
More about : WNK4 |
| Long gene name: |
WNK lysine deficient protein kinase 4 |
| Synonyms gene: |
PRKWNK4 |
| Synonyms gene name: |
lysine deficient 4 , protein kinase |
| Locus: |
17q21, 2 |
| Discovery year: |
2001-02-07 |
| GenBank acession: |
AJ309861 |
| Entrez gene record: |
65266 |
| Havana BLAST/BLAT: |
OTTHUMG00000180650 |