| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
AIF |
| Specificity: |
This antibody reacts specifically with AIF |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Mitochondrion, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human AIF |
| Gene ID: |
9131 |
| Swiss Prot: |
O95831 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Pig/Swine (Sus scrofa), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binds to DNA in a sequence-independent manner, In contrast, In response to apoptotic stimuli, Interacts with EIF3G, Plays a critical role in caspase-independent, The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i, and activates casapse-7 to amplify apoptosis, caspase-independent fragmentation of chromosomal DNA, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, pyknotic cell death in hydrogen peroxide-exposed cells, where it functions as a proapoptotic factor in a caspase-independent pathway, Functions both as NADH oxidoreductase and as regulator of apoptosis, and thereby inhibits the EIF3 machinery and protein synthesis, e |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AIF CMTX4 |
| Also known as: |
AIF Polyclonal Antibody |
| Other name: |
Anti-AIF Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
AIF |
| Short name: |
Anti-AIF (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-AIF (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8768 |
| Gene: |
AIFM1 |
More about : AIFM1 |
| Long gene name: |
apoptosis inducing factor mitochondria associated 1 |
| Synonyms gene: |
PDCD8 NAMSD |
| Synonyms gene name: |
1 apoptosis inducing factor, axonal, mitochondria associated 1 , mitochondrion-associated, motor-sensory with deafness and mental retardation (Cowchock syndrome) apoptosis-inducing factor, programmed cell death 8 (apoptosis-inducing factor) neuropathy |
| Locus: |
Xq26, 1 |
| Discovery year: |
1999-05-28 |
| GenBank acession: |
AF100928 |
| Entrez gene record: |
9131 |
| Pubmed identfication: |
9989411 23217327 |
| Havana BLAST/BLAT: |
OTTHUMG00000022392 |
| Locus Specific Databases: |
Mental Retardation database |