| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Cytochrome C |
| Specificity: |
This antibody reacts specifically with Cytochrome C |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Mitochondrion |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Cytochrome C |
| Gene ID: |
54205 |
| Swiss Prot: |
P99999 |
| Applications: |
IF(ICC), IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, Cytochrome c then transfers this electron to the cytochrome oxidase complex, Plays a role in apoptosis, Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol, The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase, the final protein carrier in the mitochondrial electron-transport chain, which then accelerates apoptosis by activating other caspases, Electron carrier protein |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
COX8-3 |
| Also known as: |
Cytochrome C Polyclonal Antibody |
| Other name: |
Anti-Cytochrome C Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Cytochrome C |
| Short name: |
Anti-Cytochrome C (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Cytochrome C (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
24382 |
| Gene: |
COX8C |
More about : COX8C |
| Long gene name: |
cytochrome c oxidase subunit 8C |
| Synonyms gene name: |
cytochrome c oxidase subunit VIIIC |
| Synonyms name: |
cytochrome c oxidase subunit VIII isoform 3 |
| Locus: |
14q32, 12 |
| Discovery year: |
2004-03-24 |
| GenBank acession: |
AY161004 |
| Entrez gene record: |
341947 |
| Pubmed identfication: |
12909344 |
| RefSeq identity: |
NM_182971 |
| Classification: |
Mitochondrial complex IV: cytochrome c oxidase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000171279 |