| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ASIC3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the ASIC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This ASIC3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9UHC3 |
| Purity: |
Antigen affinity |
| Description: |
(1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH, ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents, Babinski et al, By radiation hybrid analysis, De Weille et al, also known as Testis sodium channel (TNAC1) or Dorsal root acid-sensing ion channel (DRASIC), de Weille et al, is a protein that in humans is encoded by the ASIC3 gene, (1998) found that the human protein supported an H(+)-gated cation current in COS cells with kinetics similar to those of the rat protein, (1998) mapped the gene to chromosome 7q35, Acid-Sensing Ion Channel 3 |
| Immunogen: |
An amino acid sequence from the N-terminus of human Acid-Sensing Ion Channel 3 (FLYQVAERVRYYREFHHQ) was used as the immunogen for this ASIC3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ASIC3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ASIC3 |
| Short name: |
ASIC3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ASIC3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
101 |
| Gene: |
ASIC3 |
More about : ASIC3 |
| Long gene name: |
acid sensing ion channel subunit 3 |
| Synonyms gene: |
ACCN3 |
| Synonyms gene name: |
amiloride-sensitive cation channel 3, testis acid-sensing (proton-gated) ion channel 3 |
| Synonyms: |
TNaC1 DRASIC |
| Synonyms name: |
testis sodium channel 1 |
| Locus: |
7q36, 1 |
| Discovery year: |
1999-05-04 |
| GenBank acession: |
AB010575 |
| Entrez gene record: |
9311 |
| Pubmed identfication: |
9571199 9744806 |
| RefSeq identity: |
NM_004769 |
| Classification: |
Acid sensing ion channel subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000158685 |