| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Orai1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Orai1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Orai1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q96D31 |
| Purity: |
Antigen affinity |
| Description: |
Glu106-to-asp(E106D) and glu190-to-gln(E190Q) substitutions in transmembrane helices 1 and 3, Orai proteins share no homology with any other ion channel family of any other known proteins, Orai1 channels play an important role in the activation of T-lymphocytes, Orai2 and Orai3, Prakriya et al, The loss of function/mutation of Orai1 causes Severe combined immunodeficiency (SCID) in humans, The mammalian Orai family has two additional homologs, They have 4 transmembrane domains and form tetramers, They showed that Orai1 is a PM protein, also known as CRACM1, and TMEM142A, and rendered the channel permeable to cesium ion, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments, diminished calcium ion influx, increased current carried by monovalent cations, is a calcium selectiveion channel that in humans is encoded by the ORAI1 gene, respectively, (2006) showed that ORAI1 is a PM protein, Orai calcium release-activated calcium modulator 1 |
| Immunogen: |
An amino acid sequence from the C-terminus of human Orai1 (EFARLQDQLDHRGDHPLTPGSHYA) was used as the immunogen for this Orai1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Orai1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Orai1 |
| Short name: |
Orai1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Orai1 (Antibody to) |
| Alternative technique: |
antibodies |