| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SDHA |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the SDHA antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This SDHA antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P31040 |
| Purity: |
Antigen affinity |
| Description: |
Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits, In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant protein more susceptible to proteolysis, The findings indicated that SDHA, The gene that codes for the protein is nuclear, can act as a tumor suppressor gene, even though the protein is located in the inner membrane of the mitochondria, flavoprotein is a protein that in humans is encoded by the SDHA gene, like other SDH subunits, subunit a, Succinate dehydrogenase complex |
| Immunogen: |
mouse and rat), An amino acid sequence from the N-terminus of human SDHA (NMEEDNWRWHFYDTVK) was used as the immunogen for this SDHA antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the SDHA antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SDHA |
| Short name: |
SDHA Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SDHA (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10680 |
| Gene: |
SDHA |
More about : SDHA |
| Long gene name: |
succinate dehydrogenase complex flavoprotein subunit A |
| Synonyms gene: |
SDH2 |
| Synonyms gene name: |
flavoprotein (Fp) , flavoprotein (Fp) succinate dehydrogenase complex subunit A, subunit A, succinate dehydrogenase complex |
| Synonyms: |
FP SDHF |
| Synonyms name: |
succinate dehydrogenase [ubiquinone] flavoprotein subunit flavoprotein subunit of complex II |
| Locus: |
5p15, 33 |
| Discovery year: |
1995-10-24 |
| GenBank acession: |
BC001380 |
| Entrez gene record: |
6389 |
| Pubmed identfication: |
7798181 |
| RefSeq identity: |
NM_004168 |
| Classification: |
Mitochondrial complex II: succinate dehydrogenase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000090275 |
| Locus Specific Databases: |
SDHA @ LOVD TCA Cycle Gene Mutation Database LRG_315 |