| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
LIF |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the LIF antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This LIF antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P09056 |
| Purity: |
Antigen affinity |
| Description: |
It is produced in high amounts by the human endometrium and the trophoblast itself, LIF could thus play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface, The gene maps to 22q11-q12, and its receptors are present on cytotrophoblast cells, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12, 2, 2, LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice |
| Immunogen: |
An amino acid sequence from the C-terminus of mouse LIF (DKEAFQRKKLGCQLLGTYKQ) was used as the immunogen for this LIF antibody (100% rat homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the LIF antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LIF |
| Short name: |
LIF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
leukemia inhibitory factor (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CDF and DIA and HILDA and MLPLI, Extracellular, LIF and IDBG-3968 and ENSG00000128342 and 3976, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, growth factor activity, this GO :0001135 and RNA polymerase II transcription factor recruiting transcription factor activity and molecular function this GO :0001974 and blood vessel remodeling and biological process this GO :0005102 and receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005146 and leukemia inhibitory factor receptor binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006955 and immune response and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007566 and embryo implantation and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0030324 and lung development and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0033141 and positive regulation of peptidyl-serine phosphorylation of STAT protein and biological process this GO :0042503 and tyrosine phosphorylation of Stat3 protein and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0045595 and regulation of cell differentiation and biological process this GO :0045651 and positive regulation of macrophage differentiation and biological process this GO :0045835 and negative regulation of meiosis and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046697 and decidualization and biological process this GO :0046888 and negative regulation of hormone secretion and biological process this GO :0048286 and lung alveolus development and biological process this GO :0048644 and muscle organ morphogenesis and biological process this GO :0048666 and neuron development and biological process this GO :0048711 and positive regulation of astrocyte differentiation and biological process this GO :0048861 and leukemia inhibitory factor signaling pathway and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0060135 and maternal process involved in female pregnancy and biological process this GO :0060426 and lung vasculature development and biological process this GO :0060463 and lung lobe morphogenesis and biological process this GO :0060707 and trophoblast giant cell differentiation and biological process this GO :0060708 and spongiotrophoblast differentiation and biological process this GO :0070373 and negative regulation of ERK1 and ERK2 cascade and biological process this GO :0072108 and positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis and biological process this GO :0072307 and regulation of metanephric nephron tubule epithelial cell differentiation and biological process this GO :1900182 and positive regulation of protein localization to nucleus and biological process this GO :1901676 and positive regulation of histone H3-K27 acetylation and biological process, this GO :0001135 : RNA polymerase II transcription factor recruiting transcription factor activity, this GO :0001135 : RNA polymerase II transcription factor recruiting transcription factor activity and also this GO :0005102 : receptor binding and also this GO :0005125 : cytokine activity and also this GO :0005146 : leukemia inhibitory factor receptor binding and also this GO :0008083 : growth factor activity, this GO :0005102 : receptor binding, this GO :0005125 : cytokine activity, this GO :0005146 : leukemia inhibitory factor receptor binding, this GO :0008083 : growth factor activity, leukemia inhibitory factor |
| Identity: |
6596 |
| Gene: |
LIF |
More about : LIF |
| Long gene name: |
leukemia inhibitory factor |
| Synonyms: |
CDF DIA HILDA |
| Synonyms name: |
differentiation inhibitory activity differentiation-inducing factor hepatocyte-stimulating factor III cholinergic differentiation factor human interleukin in DA cells |
| Locus: |
22q12, 2 |
| Discovery year: |
1989-06-11 |
| Entrez gene record: |
3976 |
| Pubmed identfication: |
1714745 8058719 |
| RefSeq identity: |
NM_002309 |
| Classification: |
Endogenous ligands Interleukin 6 cytokine family |
| Havana BLAST/BLAT: |
OTTHUMG00000150910 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |