| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SKP2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the SKP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This SKP2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q13309 |
| Purity: |
Antigen affinity |
| Description: |
Skp2 controls p300-p53 signaling pathways in cancer cells, This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors, making it a potential molecular target for cancer therapy, such as the cell cycle inhibitor p27, The F box protein Skp2(S-phase kinase-associated protein 2) is oncogenic |
| Immunogen: |
An amino acid sequence from the N-terminus of human SKP2 (HRKHLQEIPDLSSNVATSF) was used as the immunogen for this SKP2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the SKP2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SKP2 |
| Short name: |
SKP2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SKP2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10901 |
| Gene: |
SKP2 |
More about : SKP2 |
| Long gene name: |
S-phase kinase associated protein 2 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , S-phase kinase-associated protein 2 (p45) S-phase kinase-associated protein 2 |
| Synonyms: |
FBXL1 FBL1 p45 |
| Locus: |
5p13, 2 |
| Discovery year: |
1995-12-18 |
| GenBank acession: |
U33761 |
| Entrez gene record: |
6502 |
| Pubmed identfication: |
8646875 |
| RefSeq identity: |
NM_005983 |
| Classification: |
F-box and leucine rich repeat proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000131106 |