| Also known as: |
Complement C4c |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
42289 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Binds both C4c and C4 and so is not suitable for all ELISA applications, C4b levels are strictly regulated, C4c consists of two a-chain fragments, It is expressed in the blood plasma, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a, This particular antibody might cross react with speacies outside of the listed ones, alpha 4 (13 kD) and alpha 3 (25 kD) linked to a beta chain through disulphide bridges and a gamma chain, iC4b is further degraded into the inactive fragments C4d and C4c, inhibiting opsonization and activation of the classical pathway, Recognizes complement component 4c (C4c) |
| Purification method: |
Protein A Purified |
| Form/Appearance: |
0, 09% sodium azide, Borate buffered saline |
| Concentration: |
1 miligram per 1 mililiter |
| Storage and shipping: |
For long term storage aliquot and keep the antibody at -20 degrees Celsius, Freeze-thaw cycles should be avoided in order to retain the activity of the antibody, Store undiluted, Store at +4 degrees Celsius for short term storage |
| Tested applications:: |
ELISA (EIA), Flow Cytometry (FC/FACS), IHC - Frozen), Western Blot (WB), Immunohistochemistry (IHC - Paraffin |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
Monoclonal [ 10-12] (IgG1) Complement C4c |
| Short name: |
Mouse Monoclonal [clone 10-12] (IgG1) Complement C4c Antibody |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype: |
IgG1 |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
sapiens Complement C4c (Antibody to), Mouse monoclonal [clonality 10-12] (IgG1) to H |
| Alternative technique: |
antibodies |