| Also known as: |
Gr-1 |
| Other names: |
Ly-6G, Lymphocyte antigen 6G, N/A, N/A, 1, Lymphocyte antigen 6G |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
Gr-1 |
| Other gene names: |
Ly-6G, Ly6g, N/A, N/A |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2b |
| Clone: |
RB6-8C5 |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
Clone RB6-8C5 has been reported to deplete mouse mature neutrophils <, Clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated, Expression of Gr-1 on a subpopulation of lymphocytes has also been reported, Gr-1 is also expressed on macrophages and transiently on differentiating monocytes, In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The Gr-1 antigen is primarily a marker of myeloid differentiation, This particular antibody might cross react with speacies outside of the listed ones, a 21-25kD cell surface protein that belongs to the Ly-6 family of proteins, /I>, Gr-1 This item recognises the mouse Gr-1 antigen, I>, in vivo< |
| Form/Appearance: |
Biotin (Purified IgG conjugated to Biotin - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost-free freezers is not recommended, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Biotinylated |
| Gene target: |
Gr-1: |
| Short name: |
RAT ANTI MOUSE Gr-1: Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
Biotin |
| Alternative name: |
RAT antibody to MOUSE Gr-1:biotinilated (Antibody to) |
| Alternative technique: |
antibodies |