| Long name: |
Biotin Conjugated, AE2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AE2 |
| Specificity: |
This is a highly specific antibody against AE2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human AE2/SLC4A2 |
| Gene ID number: |
6522 |
| Tested applications: |
IHC-P |
| Recommended dilutions: |
IHC-P(1:100-500) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Bicarbonate is secreted from hepatocytes and cholangiocytes through parallel mechanisms, Gene silencing of AE2 causes a marked inhibition of unstimulated and secretin-stimulated chloride/bicarbonate exchange, The AE2 protein regulates pH, The excretion of bicarbonate at both the canaliculi and the bile ducts is an important contributor to the generation of bile-salt independent flow, cell volume and transepithelial ion transport in many tissues, chloride concentration, including hormones, innervation/neuropeptides and biliary constituents, which involve chloride efflux through activation of chloride channels and further bicarbonate secretion via AE2 (also designated SLC4A2)-mediated chloride/bicarbonate exchange, which maintains the bile acid pool that is crucial for secretin to induce bicarbonate-rich choleresis, Primary canalicular bile undergoes a process of fluidization and alkalinization along the biliary tract that is influenced by several factors |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
AE2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AE2 Conjugated |
| Short name: |
Conjugated, AE2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, AE2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
25076 |
| Gene: |
NBPF3 |
More about : NBPF3 |
| Long gene name: |
neuroblastoma breakpoint family member 3 |
| Synonyms gene name: |
member 3 , neuroblastoma breakpoint family |
| Locus: |
1p36, 12 |
| Discovery year: |
2005-11-23 |
| GenBank acession: |
BC024011 |
| Entrez gene record: |
84224 |
| Pubmed identfication: |
11230166 16079250 |
| RefSeq identity: |
NM_032264 |
| Classification: |
Neuroblastoma breakpoint family |
| Havana BLAST/BLAT: |
OTTHUMG00000002944 |