| Long name: |
Biotin Conjugated, MEL18 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MEL18 |
| Specificity: |
This is a highly specific antibody against MEL18 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
170-210/344 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human MEL18 |
| Gene ID number: |
7703 |
| Swiss Prot: |
P35227 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells, Binds specifically to the DNA sequence 5'-GACTNGACT-3', Component of a Polycomb group (PcG) multiprotein PRC1-like complex, Has tumor suppressor activity, Is not functionally redundant with BMI1, May play a role in control of cell proliferation and/or neural cell development, PcG PRC1 complex acts via chromatin remodeling and modification of histones, Regulates proliferation of early T progenitor cells by maintaining expression of HES1, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility, throughout development, unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similarity), Transcriptional repressor |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
DNA-binding protein Mel-18, MEL18, PCGF2, Polycomb group RING finger protein 2, RING finger protein 110, RNF110, ZNF144, Zinc finger protein 144, MEL-18 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MEL18 Conjugated |
| Short name: |
Conjugated, MEL18 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, MEL18 (Antibody to) |
| Alternative technique: |
antibodies |