| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MEL18 |
| Specificity: |
This antibody reacts specifically with MEL18 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MEL18 |
| Gene ID: |
7703 |
| Swiss Prot: |
P35227 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells, Binds specifically to the DNA sequence 5'-GACTNGACT-3', Component of a Polycomb group (PcG) multiprotein PRC1-like complex, Has tumor suppressor activity, Is not functionally redundant with BMI1, May play a role in control of cell proliferation and/or neural cell development, PcG PRC1 complex acts via chromatin remodeling and modification of histones, Regulates proliferation of early T progenitor cells by maintaining expression of HES1, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility, throughout development, unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similarity), Transcriptional repressor |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DNA-binding protein Mel-18, MEL18, PCGF2, Polycomb group RING finger protein 2, RING finger protein 110, RNF110, ZNF144, Zinc finger protein 144, MEL-18 |
| Also known as: |
MEL18 Polyclonal Antibody |
| Other name: |
Anti-MEL18 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MEL18 |
| Short name: |
Anti-MEL18 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MEL18 (polyclonal) |
| Alternative technique: |
polyclonals |