| Long name: |
Biotin Conjugated, Midline 1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Midline-1 |
| Specificity: |
This is a highly specific antibody against Midline-1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Midline-1/RNF59 |
| Gene ID number: |
4281 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Defects in MID1 are the cause of Opitz syndrome type I (OS-I), It also interacts with IGBP1 (Lymphocyte signaling protein A4), It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2, Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation, OS-I is an X-linked recessive disorder characterized by hypertelorism, OS-I mutations produce proteins with a decreased affinity for microtubules, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1, developmental delay and congenital heart defects, genital-urinary defects such as hypospadias in males and splayed labia in females, imperforate anus, lip-palate-laryngotracheal clefts, one B30, one COS domain, one fibronectin type-III domain and one RING-type zinc finger, 2/SPRY domain, Midline-1 (Tripartite motif-containing protein 18 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
BBBG1, FXY, Finger on X and Y mouse homolog of antibody, GBBB 1, GBBB1, MID 1, MID-1, Mid1, Midin, Midline 1, Midline 1 Opitz/BBB syndrome, Midline 1 RING finger protein, Midline 1 ring finger, Midline-1, Midline1, OGS 1, OGS1, OS antibody, OSX, Putative transcription factor XPRF, RING finger protein 59, RNF 59, RNF59, TRI18, TRI18_HUMAN, TRIM 18, TRIM18, Tripartite mot containing protein 18, Tripartite mot protein TRIM18, Tripartite mot-containing protein 18, XPRF, ZNFXY, Zinc finger X and Y antibody, BBBG 1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Midline 1 Conjugated |
| Short name: |
Conjugated, Midline 1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Midline 1 (Antibody to) |
| Alternative technique: |
antibodies |