| Long name: |
FITC Conjugated, RNF180 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RNF180 |
| Specificity: |
This is a highly specific antibody against RNF180 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human RNF180 |
| Gene ID number: |
285671 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, Expressed as three alternatively spliced isoforms, Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation, RNF180 (ring finger protein 180), RNF180 is an E3 ubiquitin ligase involved in the ubiquitin-proteasome pathway, RNF180 is encoded by a gene located on human chromosome 5, RNF180 is expressed in brain, RNF180 is well conserved among vertebrates, also known as Rines, is a 592 single-pass membrane protein that contains a single RING-type zinc finger, is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome, kidney, or of chromosome 5 altogether, testis and uterus and localizes to the endoplasmic reticulum, which contains 181 million base pairs and comprises nearly 6% of the human genome, while deletion of the q arm, The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RNF180 Conjugated |
| Short name: |
FITC Conjugated, RNF180 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, RNF180 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
27752 |
| Gene: |
RNF180 |
More about : RNF180 |
| Long gene name: |
ring finger protein 180 |
| Locus: |
5q12, 3 |
| Discovery year: |
2005-01-26 |
| GenBank acession: |
AK090756 |
| Entrez gene record: |
285671 |
| RefSeq identity: |
NM_178532 |
| Classification: |
Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000162278 |