| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RNF180 |
| Specificity: |
This antibody reacts specifically with RNF180 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RNF180 |
| Gene ID: |
285671 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, Expressed as three alternatively spliced isoforms, Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation, RNF180 (ring finger protein 180), RNF180 is an E3 ubiquitin ligase involved in the ubiquitin-proteasome pathway, RNF180 is encoded by a gene located on human chromosome 5, RNF180 is expressed in brain, RNF180 is well conserved among vertebrates, also known as Rines, is a 592 single-pass membrane protein that contains a single RING-type zinc finger, is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome, kidney, or of chromosome 5 altogether, testis and uterus and localizes to the endoplasmic reticulum, which contains 181 million base pairs and comprises nearly 6% of the human genome, while deletion of the q arm, The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
RNF180 Polyclonal Antibody |
| Other name: |
Anti-RNF180 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RNF180 |
| Short name: |
Anti-RNF180 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RNF180 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
27752 |
| Gene: |
RNF180 |
More about : RNF180 |
| Long gene name: |
ring finger protein 180 |
| Locus: |
5q12, 3 |
| Discovery year: |
2005-01-26 |
| GenBank acession: |
AK090756 |
| Entrez gene record: |
285671 |
| RefSeq identity: |
NM_178532 |
| Classification: |
Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000162278 |