| Long name: |
Biotin Conjugated, Ube2L3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Ube2L3 |
| Specificity: |
This is a highly specific antibody against Ube2L3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Ube2L3 |
| Gene ID number: |
7332 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
NF-kB maturation, Specifically, The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex, The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate, The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component, UBE2E1 and UBE2L3, UBE2E1 interacts with the tumor suppressor protein TSSC5, UBE2L3 has been shown to mediate c-fos degradation, also designated UBCH6 and UBCH7 respectively in human, are E2 conjugating enzymes that interact with various proteins, human papilloma virus-mediated p53 and Myc protein degradation, The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
E2-F1, L UBC, L-UBC, UB2L3_HUMAN, UBCE7, UbcH7, UbcM4, Ube2l3, Ubiquitin carrier protein L3, Ubiquitin conjugating enzyme E2 L3, Ubiquitin protein ligase L3, Ubiquitin-conjugating enzyme E2 L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, E2 F1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Ube2L3 Conjugated |
| Short name: |
Conjugated, Ube2L3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Ube2L3 (Antibody to) |
| Alternative technique: |
antibodies |