| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ube2L3 |
| Specificity: |
This antibody reacts specifically with Ube2L3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Ube2L3 |
| Gene ID: |
7332 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
NF-kB maturation, Specifically, The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex, The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate, The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component, UBE2E1 and UBE2L3, UBE2E1 interacts with the tumor suppressor protein TSSC5, UBE2L3 has been shown to mediate c-fos degradation, also designated UBCH6 and UBCH7 respectively in human, are E2 conjugating enzymes that interact with various proteins, human papilloma virus-mediated p53 and Myc protein degradation, The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
UBCH7 |
| Also known as: |
Ube2L3 Polyclonal Antibody |
| Other name: |
Anti-Ube2L3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Ube2L3 |
| Short name: |
Anti-Ube2L3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ube2L3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12488 |
| Gene: |
UBE2L3 |
More about : UBE2L3 |
| Long gene name: |
ubiquitin conjugating enzyme E2 L3 |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2L 3 |
| Locus: |
22q11, 21 |
| Discovery year: |
1997-03-21 |
| GenBank acession: |
AJ000519 |
| Entrez gene record: |
7332 |
| Pubmed identfication: |
8672131 9693040 |
| RefSeq identity: |
NM_198157 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000150823 |