| Long name: |
Biotin Conjugated, PRAME Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PRAME |
| Specificity: |
This is a highly specific antibody against PRAME |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
180-227/509 |
| Subcellular location: |
Cell membrane, Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human PRAME |
| Gene ID number: |
23532 |
| Swiss Prot: |
P78395 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Prevents retinoic acid-induced cell proliferation arrest, RARB and RARG, differentiation and apoptosis, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, Functions as a transcriptional repressor |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
CT130 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PRAME Conjugated |
| Short name: |
Conjugated, PRAME Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, preferentially expressed antigen in melanoma (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CT130 and MAPE and OIP-4 and OIP4, DNA-templated and biological process this GO :0006915 and apoptotic process and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0030154 and cell differentiation and biological process this GO :0040008 and regulation of growth and biological process this GO :0042974 and retinoic acid receptor binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0048387 and negative regulation of retinoic acid receptor signaling pathway and biological process, PRAME and IDBG-2240 and ENSG00000185686 and 23532, nuclei, retinoic acid receptor binding, this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0042974 : retinoic acid receptor binding, this GO :0042974 : retinoic acid receptor binding, preferentially expressed antigen in melanoma |
| Identity: |
9336 |
| Gene: |
PRAME |
More about : PRAME |
| Long gene name: |
preferentially expressed antigen in melanoma |
| Synonyms gene: |
MAPE |
| Synonyms name: |
cancer/testis antigen 130 |
| Locus: |
22q11, 22 |
| Discovery year: |
1996-06-21 |
| GenBank acession: |
U65011 |
| Entrez gene record: |
23532 |
| Pubmed identfication: |
9047241 10591208 |
| RefSeq identity: |
NM_206953 |
| Classification: |
PRAME family |
| Havana BLAST/BLAT: |
OTTHUMG00000151172 |