| Long name: |
Cy3 Conjugated, MRGPRX1/3/4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MRGPRX1/3/4 |
| Specificity: |
This may preferentially target MRGPRX1 over 3 and 4 due to slightly higher sequence similarity, This is a highly specific antibody against MRGPRX1/3/4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1-35/322 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human MRGX1 |
| Gene ID number: |
117195, 117196, 259249 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells, G Alpha q proteins are involved in the calcium-signaling pathway downstream of the MRGX receptors, G(alpha)q proteins are involved in the calcium-signaling pathway, MRGX receptors are unique in that they are expressed in a subset of small dorsal root and trigeminal sensory neurons, MRGX1 and MRGX2 receptors stimulate both G Alpha q- and G Alpha i -regulated pathways, May regulate nociceptor function and/or development, Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22), Probably involved in the function of nociceptive neurons, including the sensation or modulation of pain, while MRGX3 and MRGX4 receptors mainly activate G Alpha q-regulated pathways, There are four members (MRGX1-4) in the human MRGX familly |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
MRGX1 |
| Properties: |
1 of the MRGPRX1/3/4 Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MRGPRX1/3/4 Conjugated |
| Short name: |
Conjugated, MRGPRX1/3/4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, member X1/3/4 (Antibody to), MAS-related GPR |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
G-protein coupled receptor activity, MRGPRX1 and IDBG-35377 and ENSG00000170255 and 259249, Plasma membranes, member X1, this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006953 and acute-phase response and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0016021 and integral component of membrane and cellular component, this GO :0004930 : G-protein coupled receptor activity, this GO :0004930 : G-protein coupled receptor activity, MAS-related GPR |
| Identity: |
17962 |
| Gene: |
MRGPRX1 |
More about : MRGPRX1 |
| Long gene name: |
MAS related GPR family member X1 |
| Synonyms gene name: |
MAS-related GPR, member X1 |
| Locus: |
11p15, 1 |
| Discovery year: |
2005-05-09 |
| Entrez gene record: |
259249 |
| Pubmed identfication: |
11551509 |
| RefSeq identity: |
NM_147199 |
| Classification: |
Class A orphans , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000162655 |