| Long name: |
Biotin Conjugated, MRGPRX1/3/4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MRGPRX1/3/4 |
| Specificity: |
This may preferentially target MRGPRX1 over 3 and 4 due to slightly higher sequence similarity, This is a highly specific antibody against MRGPRX1/3/4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1-35/322 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human MRGX1 |
| Gene ID number: |
117195, 117196, 259249 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells, G Alpha q proteins are involved in the calcium-signaling pathway downstream of the MRGX receptors, G(alpha)q proteins are involved in the calcium-signaling pathway, MRGX receptors are unique in that they are expressed in a subset of small dorsal root and trigeminal sensory neurons, MRGX1 and MRGX2 receptors stimulate both G Alpha q- and G Alpha i -regulated pathways, May regulate nociceptor function and/or development, Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22), Probably involved in the function of nociceptive neurons, including the sensation or modulation of pain, while MRGX3 and MRGX4 receptors mainly activate G Alpha q-regulated pathways, There are four members (MRGX1-4) in the human MRGX familly |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
MRGX1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MRGPRX1/3/4 Conjugated |
| Short name: |
Conjugated, MRGPRX1/3/4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, member X1/3/4 (Antibody to), MAS-related GPR |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
G-protein coupled receptor activity, MRGPRX1 and IDBG-35377 and ENSG00000170255 and 259249, Plasma membranes, member X1, this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006953 and acute-phase response and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0016021 and integral component of membrane and cellular component, this GO :0004930 : G-protein coupled receptor activity, this GO :0004930 : G-protein coupled receptor activity, MAS-related GPR |
| Identity: |
17962 |
| Gene: |
MRGPRX1 |
More about : MRGPRX1 |
| Long gene name: |
MAS related GPR family member X1 |
| Synonyms gene name: |
MAS-related GPR, member X1 |
| Locus: |
11p15, 1 |
| Discovery year: |
2005-05-09 |
| Entrez gene record: |
259249 |
| Pubmed identfication: |
11551509 |
| RefSeq identity: |
NM_147199 |
| Classification: |
Class A orphans , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000162655 |